ISOLATION AND AMPLIFICATION OF Anaplasma marginale IN CHICKEN EMBRYO FIBROBLAST CELL CULTURE

Anaplasma marginale (A. marginale) is a worldwide pathogen that infects variety ofruminants, but mostly cattle. The present study aimed to describe an isolation technique for A. marginale, using chicken embryo Þ broblast (CEF) cell culture. Blood and tick samples were collected from 5 calves 2 3 months old, which considered be infected with A.marginale due anemia, jaundiced mucous membranes, prostration. DNA extractionand PCR performed diagnosis blood samples. All tested positive in PCR. Additionally,ticks crushed the aid of blender inoculation CEF After inoculation, cultures kept at 37ºC 5%CO2 15 days. supernatant was again analyzed Wright stain method conÞ rm marginaleisolation. Cell PCR, presence agent demonstrated by stain. Therefore, CEFcell culture it possible isolate amplify concentration 1.3x 107.2 bodies per mL. cells are undemanding easy preserve; they anoption production under laboratory conditions.